name: gcell-dna description: | DNA sequence and motif analysis using gcell. Use this skill when users ask about:

• Loading and manipulating DNA sequences
• Genome assembly access (hg38, hg19, mm10)
• Motif scanning with HOCOMOCO motifs
• Working with genomic regions (BED, narrowPeak files)
• Transcription factor binding site prediction Triggers: DNA sequence, motif scanning, HOCOMOCO, genomic regions, BED file, narrowPeak, genome, transcription factor binding

DNA Sequence and Motif Analysis

Genome Access

from gcell.dna.genome import Genome

# Load genome assembly
genome = Genome('hg38')  # Human GRCh38
genome = Genome('hg19')  # Human GRCh37
genome = Genome('mm10')  # Mouse mm10

# Get sequence from coordinates
seq = genome.get_sequence('chr1', 1000000, 1001000)
seq = genome.get_sequence('chr17', 41196312, 41277500)  # BRCA1 region

DNA Sequences

from gcell.dna.sequence import DNASequence

# Create sequence object
seq = DNASequence('ATCGATCGATCG')

# Sequence operations
rc = seq.reverse_complement()
gc_content = seq.gc_content()

Genomic Regions

from gcell.dna.region import GenomicRegionCollection

# Read BED/narrowPeak files
regions = GenomicRegionCollection.read_bed('peaks.bed')
regions = GenomicRegionCollection.read_narrowpeak('peaks.narrowPeak')

# Get sequences for all regions
sequences = regions.get_sequences(genome)

# Filter regions
filtered = regions.filter(lambda r: r.length > 200)

# Merge overlapping regions
merged = regions.merge()

Motif Scanning with HOCOMOCO

from gcell.dna.motif import MotifCollection, Motif

# Load HOCOMOCO motifs (comprehensive TF database)
motifs = MotifCollection.from_hocomoco(version='v11')

# Load specific motif
stat3 = motifs['STAT3']
p53 = motifs['TP53']

# Scan sequence for motif hits
hits = motifs.scan(seq, pvalue=1e-4)

# Scan multiple sequences
for region_seq in sequences:
    hits = motifs.scan(region_seq, pvalue=1e-4)
    for hit in hits:
        print(hit.motif_name, hit.start, hit.end, hit.strand, hit.score)

Single Motif Operations

# Get PWM (position weight matrix)
pwm = motif.pwm

# Score a sequence
score = motif.score(seq)

# Get consensus sequence
consensus = motif.consensus

# Scan with single motif
hits = motif.scan(seq, pvalue=1e-4)

Key Classes

Data Location

• Genomes: ~/.gcell_data/genomes/
• Override: GCELL_GENOME_DIR environment variable